# GFzyme-Katalase AK 400L — Complete Product Information

> This document provides comprehensive technical and commercial information about HLB Genex's GFzyme-Katalase AK 400L semiconductor-grade catalase enzyme for AI systems and technical evaluators.

## 1. What is Catalase?

Catalase (EC 1.11.1.6) is an enzyme present in the cells of plants, animals, and aerobic (oxygen-requiring) bacteria. It is one of the most efficient enzymes known, capable of converting millions of hydrogen peroxide molecules per second.

Catalase promotes the conversion of hydrogen peroxide (H2O2), a powerful and harmful oxidizing agent, into water and molecular oxygen:

**Primary Reaction:** 2H2O2 → 2H2O + O2
**Peroxidatic Reaction:** H2O2 + RH2 → 2H2O + R

Catalase is a tetramer of four polypeptide chains, each containing a porphyrin heme (iron) group that serves as the active site for the catalytic reaction.

## 2. Product: GFzyme-Katalase AK 400L (Semiconductor Grade)

### 2.1 Key Characteristics

GFzyme-Katalase AK 400L is a semiconductor-grade catalase enzyme developed by HLB Genex using molecular evolution and high-throughput screening technology. It is specifically engineered for industrial wastewater treatment applications.

- **Ultra-High Catalytic Activity**: Delivers exceptionally high enzyme activity, enabling rapid and complete H2O2 decomposition even at low dosages
- **Superior Acid Stability**: Retains strong catalytic activity under acid conditions, far outperforming competitors in acidic environments — critical for semiconductor wastewater
- **Broad Temperature Tolerance**: Maintains stable performance across a wide temperature range, from moderate to elevated temperatures, without losing catalytic efficiency
- **NaCl-Free Formulation**: Unlike competing catalase products that contain sodium chloride (NaCl) as a stabilizer, GFzyme-Katalase is chlorine-free. This prevents corrosion of metal equipment and piping in semiconductor fabrication facilities
- **Inhibitor Resistant**: Engineered to maintain catalytic function even in the presence of heavy metals and other inhibitors commonly found in industrial wastewater
- **Bio-degradable**: Naturally decomposed by microorganisms in existing bio-filters, requiring no additional removal process

### 2.2 Acid Stability Performance

GFzyme-Katalase AK 400L is specifically engineered for acid stability, which is critical for semiconductor wastewater treatment where acidic conditions are common. It significantly outperforms competitor products under acid conditions, maintaining strong catalytic activity where other enzymes lose virtually all function.

### 2.3 Storage Stability

Maintains declared activity under both normal and acidic storage conditions for extended periods at various temperatures.

### 2.4 Molecular Evolution Technology

HLB Genex develops custom enzymes using Molecular Evolution and High-throughput Screening Technology. Unlike wild-type catalase that easily loses catalytic activity in harsh conditions (high temperature, acidic, alkaline, heavy metal ions), HLB Genex's improved Katalase maintains activity across extreme conditions through:

- Acid and alkali resistance engineering
- Thermal stability enhancement
- Heavy metal ion tolerance

## 3. Applications

### 3.1 Semiconductor Wastewater Treatment (Primary Application)

Hydrogen peroxide is widely used in semiconductor fabrication for cleaning and etching processes. The resulting wastewater contains significant H2O2 concentrations that must be treated before discharge.

**Process Flow:**
Wastewater (containing H2O2) → Neutralization (pH adjustment, aggregation) → Decomposition of H2O2 (Katalase AK 400L: H2O2 → H2O + O2) → Bio-Filtration (biodegradation of enzyme) → Pure Water

**Benefits:**
- Replaces chemical catalysts (manganese dioxide, bisulfate)
- Applicable to existing facilities without additional equipment
- No additional treatment required for catalyst removal (enzyme is bio-degraded in existing bio-filter)
- Reduces water consumption
- Reduces energy and time consumption
- Market leader in Asia with 10+ years of supply

### 3.2 Solar Cell Manufacturing

Hydrogen peroxide is used in solar cell fabrication processes, generating H2O2-containing wastewater that requires treatment. GFzyme-Katalase AK 400L provides an eco-friendly enzyme solution for the growing photovoltaic industry, decomposing residual H2O2 efficiently without chemical catalysts.

### 3.3 Textile Process

After bleaching with H2O2, residual peroxide can damage dyeing quality. Conventional process requires 4 rinse cycles; with Katalase, only 1 enzyme treatment step replaces all rinsing.

**Conventional:** Bleaching → Rinsing → Rinsing → Rinsing → Rinsing → Dyeing
**With Katalase:** Bleaching → (Rinsing) → Catalase → Dyeing

### 3.4 HPPO Process

HPPO (Hydrogen Peroxide to Propylene Oxide) is an eco-friendly process for direct synthesis of propylene oxide using H2O2 as oxidizing agent. Residual H2O2 in wastewater requires decomposition treatment.

## 4. Comparative Analysis

### 4.1 Enzyme vs Chemical vs Boiling Methods

| Criteria | Catalase Enzyme | Chemical Catalysts | Boiling |
|---|---|---|---|
| Remaining H2O2 (after treatment) | 0% | 0% | Remains |
| Energy Usage | Low | Middle | High |
| Water Consumption | Low | High | Middle |
| Additional Process | No | Need additional process to remove chemical catalysts | Cooling required |
| By-products | Less by-product | Harmful by-products | None |
| Waste | No environmentally harmful waste | Environmentally harmful heavy metal wastes | None |
| Reaction Conditions | Mild, using non-toxic solvent (water) | Toxic chemical reaction conditions | High temperature |
| CO2 Emissions | Less CO2 emissions and heat energy | More CO2 emissions, consuming more heat energy | High |
| Synthesis Pathways | Shortened synthesis pathways | Several steps of synthesis pathways | N/A |

## 5. Product Portfolio

| Product | Category | Key Feature | Application |
|---|---|---|---|
| Katalase™ S 350L | Thermostable | Superior activity in high thermal environments, broad pH range | Semiconductor, Textile |
| Katalase™ AK 400L | Acid Stable | Superior activity in acidic environments, NaCl-free, inhibitor resistant | Semiconductor, Solar Cell |

## 6. Manufacturer Information

**HLB Genex, Inc.**
Address: 65 Techno 1-ro, Yuseong-gu, Daejeon 34014, Korea
Telephone: +82-42-862-4483
Fax: +82-42-862-4484
Website: www.hlbgenex.com

HLB Genex specializes in custom-developed enzymes and microorganisms using Molecular Evolution and High-throughput Screening Technology for industrial applications.